Dissection and Fixation 1. The animal is sacrificed by cervical dislocation and the head is cut off. The brain is extracted and placed into a dissecting dish filled with 0.1[..]

Isolation and culture of neural stem cells from the adult mouse SEZ 1. The animal is sacrificed by cervical dislocation, the brain is extracted and placed into in a 60-mm[..]

1. The pup is sacrificed by cutting the head off instantaneously, the brain is extracted and placed into in a 30-mm Petri dish with ice-cold dissection medium (10mM of HEPES[..]

ChIP protocol for NS cells Chromatin Preparation Aspirate the medium from a confluent 60mm plate and wash cells with PBS. Add 400μl Accutase and incubate 2-3min at 37oC. Collect the[..]

The size of the mitotic spindle during mitosis can give us information about the type of the division. During active neurogenesis Apical Radial Glia cells perform two types of divisions[..]